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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 31.1 (CX31.1.1, Gap Junction Beta 5 Protein, CXB-5)

Anti -Connexin 31.1 (CX31.1.1, Gap Junction Beta 5 Protein, CXB-5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP
Catalog #C7853-61A
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against this epitope has been used for westerns (3).
ELISA (1100K using 50-100ng CX31.1 control peptide/well.
Immunoprecipitations. We recommend the use of affinity pure antibodies at 2-10ug affinity pure antibodies. An antibody made to a similar epitope has been used for Immunoprecipitations (3). A similar protocol is recommended for this antibody.
Immunohistochemistry & Immunofluorescence we recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue (3).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenA 14 amino acid peptide sequence within the 3rd cytoplasmic domain of the rat Cx31.1 (2) was used to generate anti-rat Cx31.1. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
SpecificityThe Cx31.1 immunogenic peptide sequence is 86% (12/14 aa) homologous with mouse CX31.1 (4). The Cx31.1 peptide sequence does not have significant homology with other known Connexins. Actual crossreactivity of antibodies in various species is not established
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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