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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)

Anti -Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #C7853-61E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3).
ELISA: 1:100K using 50-100ng CX31 control peptide/well.
Optimal dilutions to be determined by the researcher.
Control PeptideC7853-61D Connexin 31, Mouse, Control Peptide (CX31, Gap Junction Beta 3 Protein, CXB-3).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 17 amino acid peptide sequence within the 2nd cytoplasmic domain of the rat Cx31 (KLH).
SpecificityRecognizes mouse Connexin 31. Species sequence homology: shows 3 aa differences in rat Cx 31. The Cx31 peptide sequence does not significant homology with other known Connexins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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