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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 32

Anti -Connexin 32

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of lowmolecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length(1,2) Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane(3). Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis(3,4).
Catalog #C7854-05
ApplicationsSuitable for use in Immunofluorescence, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 10ug/ml
Western Blotting: 1-3ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G104
HostMouse
SourceMouse
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminus of mouse
SpecificityReacts with both the monomer and dimer forms of Connexin 32 (molecular weights of ~32kD and 51kD). Reactivity is con rmed with brain and liver from rat and mouse. Based on sequence homology, this antibody is expected to react with human Connexin 32.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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