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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)

Anti -Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver
Protein)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Catalog #C7854-05D
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against this epitope has been used for Westerns (3) to detect a broad band of 28-30kD. Additional high MW. bands (aggregated Cx32) may also be detected.
ELISA (1100K using 50-100ng Cx32 control peptide/well.
Immunohistochemistry & Immunofluorescence We recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue as described (3).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenA 19 amino acid peptide sequence within the 2nd cytoplasmic domain of the rat Cx32 (2) was used to generate anti-rat Cx32. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
SpecificityRat Cx32 immunogenic peptide sequences is the same in mouse, rat and human (4
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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