Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)

Anti -Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver
Protein)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of lowmolecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.
Catalog #C7854-05N
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-1ug/ml detects Connexin 32 in cells and tissues.
Immunohistochemistry (Frozen): 1-2ug/ml detects Connexin 32 in formalin and acetone fixed tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K172
HostMouse
SourceHuman
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa105-123 from human connexin-32. Conjugated to KLH.
SpecificityRecognizes human Connexin 32. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links