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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -Connexin 37(CX37, Gap Junction Alpha 4 Protein, CXA-4)

Anti -Connexin 37(CX37, Gap Junction Alpha 4 Protein, CXA-4)


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Clone Host Grade Applications
Polyclonal Sheep Serum B IF
Catalog #C7855-35A
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. SUBUNIT A connexon is composed of a hexamer of connexins.
Cellular LocalizationMembrane; multi-pass membrane protein.
Tissue SpecificityHighly expressed in lung. Belongs to the connexin family. Alpha-type (group II) subfamily.
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Clone TypePolyclonal
FormSupplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
ImmunogenA synthetic peptide (YLPMGE GPSSPPCPTY) as part of rat Connexin-37 protein (aa: 265-280) conjugated to diphtheria toxoid.
SpecificityWestern blotting of cell membarnes of COS cells transiently transfected with cDNA encoding Connexin-37 shows two bands of molecular weight 36 and 37kD which are abolished by preincubation with the immunising peptide. No such bands are detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-40, Connexin-43 or Connexin-45. Species Crossreactivity: This antiserum recognizes rat and mouse Connexin-37 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGap junction alpha-4 protein, Cx37, Gja4, Cxn-37, CXA-4

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