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Abs to Connexin, Junction Proteins
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Anti -Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)
Anti -Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| Rat Connexin 37 is A 333 aa gap junction protein with a predicted MW. Of ~37kD. It is prominently expressed in lung (see reviews in refs. 1). A 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of rat Cx37 (2) was used to generate anti-Mouse Cx37. Peptide was coupled to KLH (carrier protein) Via the N-terminal Cysteine and antibodies generated in rabbits. | | | Catalog # | C7855-38 | | Applications | Suitable for use in ELISA, Western Blotting. Other applications not tested. | | Recommended Dilution | Western Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique. | | ELISA (1 | 10K-100K using 50-100ng control peptide/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not Determined | | Form | Supplied as a liquid, 0.05% sodium azide, 40% glycerol. | | Purity | Serum | | Immunogen | Synthetic peptide (19aa) near the C-terminus of rat CX37 (KLH). | | Specificity | Recognizes rat Cx37. No significant homology is seen with other Connexins. Species Sequence Homology: mouse, 94%; human, 52%. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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