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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)

Anti -Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #C7855-39
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique.
ELISA (1:10K-100K using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, before the addition of 40% glycerol.
PurityPurified using a peptide-Sepharose column.
ImmunogenA 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of rat Cx37 (2) was used to generate anti-Mouse Cx37. Peptide was coupled to KLH (carrier protein) Via the N-terminal Cysteine
SpecificityRat immunogenic peptide sequence is specific for Cx37 and no significant homology is seen with other Connexin. It shows 95% and 52% sequence homology with the mouse and human Cx37, respectively (3). For human Cx37, we recommend the use of CX37A11 (antibody # 1) that is raised to a more conserved peptide. Antibody crossreactivity in various species is not studies. Control peptide, because of the small size of ~2-3kD, is not recommended for Western. It should be used in ELISA or for antibody blocking experiments to establish antibody specificity in Western, IHC or other immunoassays
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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