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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 40 (CX40, Gap Junction alpha 5 Protein, CXA-5)

Anti -Connexin 40 (CX40, Gap Junction alpha 5 Protein, CXA-5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP
Catalog #C7856-02
ApplicationsSuitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique. An antibody made to a similar epitope has been shown to recognize 40kD band in various tissues (3).
ELISA (110K-100K using 50-100ng Cx40 control peptide/well.
Immunoprecipitations. We recommend the use of affinity pure antibodies at 2-10ug affinity pure antibodies (3).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenA 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of Mouse Cx40 (2) Peptide was coupled to KLH (carrier protein)
SpecificityMouse Cx40 immunogenic peptide sequence is specific for Cx40 and no significant homology is seen with other Connexin. The Cx40 peptide is 100% (19/19 aa); 100% (19/19 aa) and 68% (13/19 aa) with the rat Cx40, human Cx 40 and chicken Cx42, respectively (4). Interspecies crossreactivity of the ant-mouse Cx40 is not known
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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