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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Connexin 43 is a member of a large family of gap junction proteins. Each gap junction is composed of two hemichannels, or connexons that provide a route for the diffusion of low molecular weight materials from cell to cell.. Connexin 43 is found in the heart and brain and is known to bind tubulin at its C-terminus.
Catalog #C7857-02
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.5ug/ml on mouse heart lysate and 1ug/ml on human heart lystate
Immunohistochemistry: 1:50. Mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0 using high heat epitope retrieval techniques.
Optimal dilutions to be determined by researcher.
Positive ControlsMouse and human heart lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa360-382 within the C-terminus of mouse connexin 43, conjugated to KLH. (Accession #NP_034418).
SpecificityRecognizes mouse Cx43 at ~43kD. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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