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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC IF
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1kD. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junctions play an important role for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junctions may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane domains: 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N- and C-termini are predicted to be cytoplasmic.
Catalog #C7857-05
ApplicationsSuitable for use in Western Blot, ELISA, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:250
Immunohistochemistry:1:2501:1000; 2% paraformaldehyde fixation of 12um cryosections of canine Purkinje fibers with incubation for 16 hours at 4°C prior to fluorescence detection.
Immunocytochemistry: 1:501:300; acetone fixation
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q251
FormSupplied as a liquid in PBS, 50% glycerol, BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa252-270 of the native sequence from rat cardiac connexin 43 (NH2-GDLSPSKDCGSPKYAYFNGK-COOH)
SpecificityRecognizes Connexin 43 (43-47kD) in mouse fibroblasts. The molecular weight range likely represents post-translational modifications of a 43kD primary translation product. Also detects Cx43 in human fibroblasts, rat brain, canine ventricular myocytes and goldfish astrocytes.
Species CrossreactivityMouse, human, rat, canine and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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