| GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), a 43kDa protein, is a protein kinase C (PKC) substrate that is expressed in the growth cone membrane during nervous system development. As the brain matures, GAP-43 expression decreases; regions of synaptic plasticity however, maintain expression. GAP-43 has been shown to play an important role in neuronal pathfinding, particularly in sites where axons must choose between two almost |
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| identical paths. The presence of GAP-43 in growing cells influences the behavior of those cells, perhaps by affecting the behavior of the actin cytoskeleton; phosphorylation can affect this activity. GAP-43 is translocated from the cytosol to adherent membrane patches by basic fibroblast growth factor (bFGF) and is phosphorylated at the same time at the PKC site (Ser |
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| 41). The N-terminal region contains the aforementioned phosphorylation site, as well as a calmodulin binding site, palmitoylation sites for membrane attachment and a region that regulates GTPgamma S binding. |
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| Catalog # | C7857-06A2 |
| Applications | Suitable for use in Western Blot. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Rat |
| Concentration | As reported |
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA and 50% glycerol |
| Purity | Purified by epitope affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to amino acid residues surrounding the phosphorylated Ser 41 of Rat GAP-43 |
| Specificity | Reacts with GAP-43 when phosphorylated at Ser 41. On Western blots, it identifies an ~48kDa band. An additional band (~70kDa) is present in Western blots of rat brain. Reactivity has been confirmed with rat brain. Reactivity with other species has not been confirmed. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
