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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Mouse connexin 43 is a 382 amino acid gap junction protein with a predicted MW ~43kD. It is prominently expressed in heart. Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1kD. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane domains: 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.
Catalog #C7857-07F
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml. Detects a band at ~43kD Cx43 in heart and other tissues (3). Higher MW bands may also be detected depending upon the samples (3).
ELISA: 1:10,000-1:100,000 using 50-100ng Cx43A control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide C7857-07G
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a 19 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse Cx43 (KLH conjugated).
SpecificityRecognizes Cx43. No significant homology is seen with other connexins. Species sequence homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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