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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

Anti -Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family which exhibit similar structural features including a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. The two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane segments and the carboxy-terminal tail. These domains are thought to mediate connexin-type specific properties including phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.
Catalog #C7857-08
ApplicationsSuitable for use in ELISA, Western Blot, and Immunoflourescence. Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml (Rat)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G107
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from rat Connexin 43.
SpecificityReacts with both phosphorylated and non-phosphorylated forms of Connexin 43. Does not detect the Connexin 43 dimer. Detects non-phosphorylated forms migrating at ~41kD and phosphorylated forms migrating between 43kD and 45kD. Species Crossreactivity: Rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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