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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 45 (Cx45, CX45, Gap Junction gamma-1 Protein, GJC1, Gap Junction alpha-7 Protein, GJA7)

Anti -Connexin 45 (Cx45, CX45, Gap Junction gamma-1 Protein, GJC1, Gap Junction alpha-7
Protein, GJA7)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Serum B IF
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.
Catalog #C7858-01A
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:10,000-1:20,000
Western Blot: 1:20,000-1:50,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
PuritySerum
ImmunogenA synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa354-367) conjugated to diphtheria toxoid. Cellular Localization: Membrane; multi-pass membrane protein. Belongs to the connexin family, alpha-type (group II) subfamily.
SpecificityRecognizes human Connexin 45 at 47kD. Band is abolished by preincubation with the immunizing peptide. Does not react in Western Blot with membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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