Anti -Connexin 45 (GJC1, Gap Junction protein, gamma 1, 45kD, Cx45, CX45, DKFZp686P0738,
Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH IF |
|
| Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Dalton. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic. | | | Catalog # | C7858-05D | | Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-10ug/ml using ECL. | | ELISA: 1:100,000 using 50-100ng control peptide/well. | | Immunofluorescence (Frozen): 2-10ug/ml | | Immunohistochemistry: 2-10ug/ml | | Optimal dilutions to be determined by the researcher. | | Recommended Control Peptide | C7858-05E: Connexin 45, Mouse (CX45, Gap Junction Alpha 6 Protein, CXA-6) (Control Peptide) | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | 1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to 14aa from mouse Connexin 45 conjugated to KLH. Species Sequence Homology | | Specificity | Recognizes mouse Connexin 45. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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