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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)

Anti -Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Connexin 46 (Cx46), also known as Gap Junction Protein Alpha-3 (GJA3), CAE3, CX46 and C2P3, maps to human chromosome 13q11-q12 and encodes a 46kD protein. Cx46, along with Cx50, is principally expressed in the lens of the eye. Cx46 mediates intercellular interactions during development and is necessary for the survival of neural crest cells. Cx46 forms gap junctions that connect lens fiber cells and are crucial for maintaining lens transparency and normal lens function. Mutations of Cx46 result in severe cataracts of the lens.
Catalog #C7858-07C
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-3ug/ml. Identifies a single band at ~46kD
Immunofluorescence: 3ug/ml
Immunohistochemistry (Frozen): 3ug/ml
Note: For IHC, mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to an internal region of the mouse Connexin 46 protein.
SpecificityRecognizes mouse Connexin 46 protein. Species Sequence Homology: rat, Chinese hamster and Golden hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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