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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 50

Anti -Connexin 50

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IP IF
Gap junctions are specialized structures formed between plasma membranes of adjacent cells that play important roles in coupling chemical and electrical activity in nearly all tissue types and early vertebrate embryos. Within gap junctions numerous proteinaceous gap junction channels, approximately 1.5nm in diameter, provide an aqueous route for the free passage of inorganic ions and small molecules (<1200D) between cell cytoplasms. These channels allow for the ef cient propagation of electrical signals and the transmission of signals carried by small regulatory molecules such as cAMP. Gap junction channels form from two opposing hemichannels, or connexons, on adjacent cells. Each connexon is comprised of a ring of six identical protein subunits, termed connexins. Connexin proteins themselves form a large multigene family. To date at least twelve highly homologous, but distinct connexin genes have been identi ed in vertebrates, each with its own distinctive, though sometimes overlapping, tissue distribution. Regions of sequence divergence, including the cytoplasmic tail, are thought to mediate connexin type-speci c properties including phosphorylation, response to gating stimuli, and membrane turnover.
Catalog #C7859-50
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.11.0ug/ml
Western Blotting: 15ug/ml
Immuno uorescence 1050ug/ml
Immunoprecipitation: 510ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM,k
Clone No3G108
HostMouse
SourceSheep
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PuritySize exclusion chromatography.
ImmunogenUrea/alkali stripped sheep lens membranes.
SpecificitySpeci c for Connexin 50. It does not crossreact with other Connexin proteins. Anti-Connexin 50 recognizes an epitope in the cytoplasmic (carboxy) tail located between residues 290440. Reacts with Connexin 50 from sheep, human, mouse, rat and probably other species. Tested in a wide variety of tissues including lens, cornea and heart valve.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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