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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Contactin 2 (Transient Axonal Glycoprotein 1, TAG1, CNTN2)

Anti -Contactin 2 (Transient Axonal Glycoprotein 1, TAG1, CNTN2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Contactin-2, also known as transient axonal glycoprotein 1 (TAG-1), is a 135kD GPI-anchored protein that is expressed on neurons and glial cells. The extracellular domain of contactin-2 consists of six Ig-like C2-set domains followed by four fibronectin type III domains. Contactin-2 is required for axonal migration and interacts homophilically at juxtaparanodal regions on myelinated fibers. Within the ECD, human Contactin-2 shares 93% aa sequence identity with mouse and rat Contactin-2.
Catalog #C7865-21
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Contactin-2 (rhCNTN2; aa 29-1012: Accession # NP_005067).
ApplicationsWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human CNTN2. Using a colorimetric detection system, the detection limit for rhCNTN2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with will increase sensitivity by 5 to 50 fold. In this application, the use of human CNTN2 monoclonal antibody is recommended.
Immunohistochemistry: This antibody was used at a concentration of 25ug/ml with appropriate secondary reagents and basic antigen retrieval to detect CNTN2 in paraffin- embedded normal human hippocampus tissue sections.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CNTN2. The detection limit for rhCNTN2 is approximately 10 ng/well. Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H156
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rhCNTN2
SpecificitySelected for its ability to detect human CNTN2 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhCNTN4, rhCNTN5, or rmCNTN3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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