Anti -CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH IC |
|
| Catalog # | C7900-80 | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue. | | Immunoprecipitation: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate. | | Immunocytochemistry: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutes | | Immunohistochemistry: Reported to detect CoREST in tissue sections. | | Included Positive Control | A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | GST fusion protein corresponding to residues 109-293 of human CoREST. | | Specificity | Recognizes human CoREST, Mr ~66kD. Species Crossreactivity: Mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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