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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1)

Anti -CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC
Catalog #C7900-80
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue.
Immunoprecipitation: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate.
Immunocytochemistry: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutes
Immunohistochemistry: Reported to detect CoREST in tissue sections.
Included Positive ControlA0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenGST fusion protein corresponding to residues 109-293 of human CoREST.
SpecificityRecognizes human CoREST, Mr ~66kD. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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