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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Cortactin (p80,85)

Anti -Cortactin (p80,85)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Cortactin is present as a p80 and p85kD protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally a SH3 domain.
Catalog #C7901-80
ApplicationsSuitable for use in Western Blot, Immunoprecipation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-2ug/ml detects Cortactin (p80/85) in mouse 3T3/A31 cell lysates. Detects Cortactin in chicken fibroblast src-transformed cell lysate. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7901-80. Proteins were visualized using an IgG (HRP) Gt x Mo and a chemiluminescence detection system.
Immunoprecipitation: Use 5ug for ~500ug of cell lysate.
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma x AJ mouse splenocytes
Included Positive Antigen ControlF4209: 3T3/A31 cell lysate. Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane.
Note: PC-12 cell lysate, HeLa whole cell lysate or A431 whole cell lysate can also be used as a positive control.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No0.T.21
FormSupplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenMixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60 src.
SpecificityRecognizes 80kD and 85kD Cortactin protein in chicken and avian cells. Recognizes 80kD protein in rodent and human cells.
Species CrossreactivityMouse, rat, hamster and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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