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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Cortactin (p80/85 src substrate, Src substrate cortactin, Amplaxin, CTTN, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1)

Anti -Cortactin (p80/85 src substrate, Src substrate cortactin, Amplaxin, CTTN, EMS1,
Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.
Catalog #C7901-83F
Cellular Localization Cytoplasmic
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide with sequence WKASAGHAVSIA representing the N terminus of the human protein (residues 2-13) according to NP_005222.
SpecificitySpecies Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesp80/85 src substrate, Src substrate cortactin, Amplaxin, CTTN, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1


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