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Specifications

The glucocorticoid Corticosterone is secreted by the adrenal gland in response to stress for mammalian species. Glucocorticoids also serve a function in the metabolism of fats, protein and carbohydrate and can be adminstered as an anti-inflammatory agent. Levels of both cortisol and corticosterone increase in mammalian species due to stressors. Only the corticosterone is the dominant glucocorticoid elevated in response to stress for both mice and rats as well as other non-human mammalian species. The actual stressors are not always indicative for the elevated levels of corticosterone but among them are climate changes, changes in environment, rapid temperature changes and changes in daily activity.
Catalog #	C7903-30
	This ELISA was developed for the quantitation of corticosterone in relation to stressors on a test subject. This ELISA is specific for corticosterone with minimal cross reactivity from other key glucocorticoids. Measurement of cortcosterone is obtained with a competitive ELISA design. The corticosterone from the sample competes for a limited number of binding sites with the enzye conjugate. Readings can be taken at 650nm or 450nm with stop solution.
Kit Components	C7903-30A: Microplate, 1x96 wells
	C7903-30C: Corticosterone Standard, 1x100ul
	C7903-30D: Corticosterone (HRP), 1x150ul
	C7903-30E: Substrate (TMB), 1x20ml
	C7903-30F: ELISA Buffer, 1x30ml
	C7903-30G: Wash Buffer (10X), 1x20ml
	C7903-30H: Extraction Buffer (5X), 1x30ml
Storage and Stability	Store at 4°C. Stable for 6 months.
CAS Number	50-22-6
Molecular Formula	C21H30O4
Crossreactivity
Corticosterone 100%
Deoxycorticosterone 38%
6-Hydroxycorticosterone 19%
Progesterone 5.1%
Tetrahydorcorticosterone 2.7%
Prednisolone 1.5%
Cortisol 1.1%
Pregnenolone 0.85%
11-Epicorticosterone 0.78%
Cortisone 0.27%
21-Desoxycortisol 0.24%
d-Aldosterone 0.13%
Testosterone 0.12%
17a-Hydroxyprogesterone
0.12%
Prednisone 0.1%
Dexamethasone 0.03%
Cholesterol < 0.01%
Estradiol < 0.01%
Estriol < 0.01%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.