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You are here:Home » Antibodies » Abs to Hormones, Steroids » Anti -CRHR1, aa107-117 (Corticotropin releasing hormone receptor 1, CRF-R, CRF1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor)

Anti -CRHR1, aa107-117 (Corticotropin releasing hormone receptor 1, CRF-R, CRF1, CRH-R1h,
CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane
helix receptor)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Catalog #C7903-79C
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence. Other applications not tested.
Recommended DilutionPeptide ELISA: 1:32,000.
Western Blot: Approx 60-70kD band observed in human brain (cerebellum) and human colon lysates.
Immunofluorescence: mouse brain.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See C7903-79C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide from the C-terminus of aa-NEEKKSKVHYH
SpecificityRecognizes human CRHR1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCRHR1; corticotropin releasing hormone receptor 1; CRF-R; CRF1; CRFR1; CRH-R1h; CRHR; CRHR1f; corticotropin releasing hormone receptor variant 1h; seven transmembrane helix receptor


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