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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -Corticotropin Releasing Hormone Binding Protein (CRHBP)

Anti -Corticotropin Releasing Hormone Binding Protein (CRHBP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Human CRHBP is a 37kD secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41kD complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.
Catalog #C7903-99A
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Cortieotrophin-Releasing Hormone Binding Protein (rhCRHBP; aa 25-322: Accession # CAA51052).
ApplicationsWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human CRHBP. Using a colorimetric detection system, the detection limit for rhCRHBP is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CRHBP. The detection limit for rhCRHBP is approximately 15 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H201
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rhCRHBP
SpecificitySelected for its ability to detect human CRHBP in direct ELISAs and Western Blots. In these applications, this antibody shows no crossreactivity with rmCRHBP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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