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You are here:Home » Molecular Biology » MB-Hormones, Steroids » Cortisol 3 CMO (BSA) (Hydrocortisone-3-CMO BSA, 4-Pregnen-11b,17,21-triol-3,20-dione 3-Carboxymethyloxime BSA)

Cortisol 3 CMO (BSA) (Hydrocortisone-3-CMO BSA, 4-Pregnen-11b,17,21-triol-3,20-dione
3-Carboxymethyloxime BSA)


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Nomenclature: Hydrocortisone-3-CMO-BSA; 4-Pregnen-11b,17,21-triol-3,20-dione 3-Carboxymethyloxime-BSA
11b, 17a, 21-Trihydroy-4-Pregnene-3, 20-dione 3-(0-carboxymethyl)oxime
Catalog #C7904-14
Solubility of BSA ConjugatesHaptens with ~30 steroids per molecule are designed for raising antibodies. They are virtually insoluble in water and buffer without detergents. The lack of solubility in water arises from both the steroid content and crosslinking. We suggest solubilization with a detergent with vigorous stirring, for example Freund's adjuvant (which contains a detergent) works well. Place the steroid onto the solution, not the other way round.
Dissolving BSA conjugates in water for cell culture experiments with molar ratios of greater than 10 moles of steroid per mole of BSA is almost impossible.
Storage and StabiltyLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight76.3kD
PurityOne spot (homogenous) when tested by TLC.
FormSupplied as a lyophilized powder. Molar Ratio
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHydrocortisone-3-CMO-BSA; 4-Pregnen-11b,17,21-triol-3,20-dione 3-Carboxymethyloxime-BSA

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