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You are here:Home » Molecular Biology » MB-Drugs » Cotinine (HRP)

Cotinine (HRP)

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Specifications

Cotinine is an alkaloid found in tobacco and is also a metabolite of nicotine. The word "cotinine" is an anagram of "nicotine". Cotinine has an in vivo half-life of approximately 20 hours, and is typically detectable for several days (up to one week) after the use of tobacco. The level of cotinine in the blood is proportionate to the amount of exposure to tobacco smoke, so it is a valuable indicator of tobacco smoke exposure, including secondary (passive) smoke.[3] People who smoke menthol cigarettes may retain cotinine in the blood for a longer period because menthol can compete with enzymatic metabolism of cotinine.[4] Genetic encoding of liver enzymes may also play a role.) It is used as a biomarker for exposure to tobacco smoke and has also been sold as an antidepressant under the brand name Scotine.
Catalog #C7904-50F
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
ConcentrationNot determined
FormSupplied as a liquid. Labeled with horseradish peroxidase (HRP).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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