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You are here:Home » Antibodies » Abs to Enzymes, Cyclooxygenase (COX) » Anti -COX 1 (PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase)

Anti -COX 1 (PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1,
prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase),
prostaglandin G/H synthase and cyclooxygenase)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Catalog #C7904-73C
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilutions
Peptide ELISA: 1:8000
Western Blot: 0.3-1ug/ml ~626kDa plus 29kDa bands observed in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See C7904-73C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenC-QDDGPAVERPSTEL, C Terminus
SpecificityThis antibody is expected to recognize both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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