COX 1, Mouse, cDNA Probe (Cyclooxygenase-1, PGHS-1, Prostaglandin Endoperoxide Synthase-1,
PHS 1, Ovarian Cancer Marker)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 (also called PGHS-1; PHS-1; Prostaglandin-endoperoxide synthase-1) and COX2 (also called PGHS-2; Prostaglandin-endoproxide synthase-2 and PHSII). Both forms of COX proteins are membrane associated heme proteins containing cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti inflammatory drugs) such as aspirin. | | | Catalog # | C7904-75M | | COX1 is composed of 70KD subunits (1) and is constitutively expressed although significant enhancement of COX1 expression can be induced in some cell types. High expression is observed in gastrointestinal tissues. | | The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids, is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2. Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1, a constitutively expressed isoform, produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible and rapidly upregulated at inflamation sites and forms proinflamatory prostanoids. The overexpression of COX-2 also leads to tumerogenesis. Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kDa in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity. The nonsteroidal antiinflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3), This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2. | | Size of the cDNA | 1.7kB | | Applications | This cDNA can be used for the synthesis, by standard random priming or nick translation techniques, of radiolabeled probes for investigations of pCox-1 gene expression. This hybridizes to Cox-1 mRNA on northern blot 2.7-3.0kB under high stringency conditions. In low stringency conditions it may hybridize with Cox-2. | | Storage | At least 1 year at –70°C. Avoid repeated freeze and thaw. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in TE buffer. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
