Suitable for use in Western Blot. Other applications not tested.
Recommended dilution
Western Blot: 1:200 (ECL) Optimum dilutions to be determined by researcher.
Notes
1. Incubate with primary antibody for 2 hours and secondary antibody for 1 hour. 2. Exposure time is 2 minutes. 3. At lower dilutions using alkaline phosphatase as secondary antibody, may cross-react with BSA.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a unique C-terminal peptide of human PGHS-2 conjugated to KLH
Form
Supplied as a liquid in 0.09% sodium azide.
Purity
Ascites
Specificity
Recognizes human PGHS-2.
References
Lee, S.H. et al. Selective Expression of mitogen-Inducible Cyclooxygenase in macrophages Stimulated with Liposaccharide. J. Biol. Chem. 1992.
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