Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Cyclooxygenase (COX) » Anti -COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Anti -COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Cyclooxygenases 1 and 2 (COX-1, COX-2) are enzymes involved in the conversion of arichidonate to prostaglandins. COX-1 and COX-2 are very similar in structure and function, though they vary in expression. COX-1 is normally expressed in most cell types, whereas COX-2 expression is at low levels unless induced by hormonal stimuli. The apparent molecular weight of COX-2 appears to be around 70kD.
COX-2 is the inducible isoform of cyclooxygenase. COX-2 is upregulated in response to number of mediators, including growth factors, cytokines and endotoxins. COX-2 appears to be expressed in several different types of human cancers, including sporadic coloreactal, breast, pancreatic and cervical cancers. COX-2 immunoreactivity is also present in neurons at postsynaptic sites in rat cerebral cortex. Besides its role in carcinogenesis, COX-2 is also involved in apoptosis, cellular adhesion as well as angiogenesis.
Catalog #C7904-83DE
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 10-20ug/ml. Formalin-fixed, paraffin-embedded tissue sections require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, prior to staining.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A53
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography
ImmunogenSynthetic peptide derived from COX-2.
SpecificityRecognizes human COX-2 protein at ~70kD. Reactivity has been confirmed by Western Blot analysis with stimulated human endothelial cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links