Anti -COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| Cyclooxygenases 1 and 2 (COX-1, COX-2) are enzymes involved in the conversion of arichidonate to prostaglandins. COX-1 and COX-2 are very similar in structure and function, though they vary in expression. COX-1 is normally expressed in most cell types, whereas COX-2 expression is at low levels unless induced by hormonal stimuli. The apparent molecular weight of COX-2 appears to be around 70kD. | | | COX-2 is the inducible isoform of cyclooxygenase. COX-2 is upregulated in response to number of mediators, including growth factors, cytokines and endotoxins. COX-2 appears to be expressed in several different types of human cancers, including sporadic coloreactal, breast, pancreatic and cervical cancers. COX-2 immunoreactivity is also present in neurons at postsynaptic sites in rat cerebral cortex. Besides its role in carcinogenesis, COX-2 is also involved in apoptosis, cellular adhesion as well as angiogenesis. | | | Catalog # | C7904-83DE | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | ELISA: 0.1-1ug/ml | | Western Blot: 1-3ug/ml | | Immunohistochemistry: 10-20ug/ml. Formalin-fixed, paraffin-embedded tissue sections require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, prior to staining. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6A53 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography | | Immunogen | Synthetic peptide derived from COX-2. | | Specificity | Recognizes human COX-2 protein at ~70kD. Reactivity has been confirmed by Western Blot analysis with stimulated human endothelial cell lysates. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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