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You are here:Home » Antibodies » Abs to Enzymes, Cyclooxygenase (COX) » Anti -COX 2, Human (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Anti -COX 2, Human (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS
2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2. Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1 is a constitutively expressed isoform. it produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible. it is rapidly upregulated at inflamation sites. It forms proinflamatory prostanoids. The overexpression of COX2 also leads to tumerogenesis. Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kD in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity. The nonsteroidal anti-inflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3). This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.
COX2 is a 74kD protein having 60% homology with COX1. It is expressed by extracellular stimuli such as tumor promoters, pro-inflamatory cytokines, mitogensand oncogenes in different cells. The control of COX2 gene expression is regulated at the level of transcriptional and post-transcriptional mechanisms.
Catalog #C7904-84G
Applications Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1-10ug/ml (ECL).
ELISA: 1:5000-1:50,000
Optimal dilutions to be determined by researcher.
Control AntigenC7904-84: COX 2, Human (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) Western Blot Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4k1
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHighly purified human COX 2.
SpecificityRecognizes COX 2. Does not crossreact with COX 1. Species Crossreactivity: human and murine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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