| Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water. |
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| Antibodies to this protein make an effective loading control for mitochondria. COXIV (COX4 )is generally expressed at a consistently high level. However, be aware that many proteins run at the same molecular weight as COXIV. Some caution is required when using antibodies to this protein as a loading control, as COXIV expression can vary under some manipulations. |
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| Catalog # | C7904-89C |
| Cellular Localization | Mitochondrion, mito inner membrane |
| Positive Control | HEK 293 lysates, Hela lysates |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic peptide made to an internal region of the human COX41 protein (within residues 1-100). [Swiss-Prot# P13073]. Homology: 100% human, 99% mouse and cow, 92% monkey, 90% rat, and 76% Zebrafish |
| Specificity | This antibody is specific for COX41. Species Crossreactivity: This antibody reacts with the human COX41 protein. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | Cytochrome C oxidase subunit IV isoform 1, mitochondrial EC=1.9.3.1, COX IV-1, Cytochrome C oxidase polypeptide IV, COX4l1, COX4, cox 4-1, COX4-1 |
