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You are here:Home » Antibodies » Abs to Hormones, Steroids » Anti -C-Peptide (Hyperproinsulinemia, Insulin Precursor, IRDN, Proinsulin)

Anti -C-Peptide (Hyperproinsulinemia, Insulin Precursor, IRDN, Proinsulin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
C-peptide is a 31 amino acid peptide released when proinsulin is split into insulin and C-peptide. In rats, two isoforms of proinsulin exists, the difference is two amino acid residues in the C-peptide, termed C-peptide I and C-peptide II. Proinsulin is split when it is released from the pancreas into the blood in response to a rise in serum glucose levels. C-peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2+ concentration and subsequent stimulation of N+, K+-ATPase and endothelial nitric oxide synthase activities. C-peptide also functions in repair of the muscular layer of arteries.
Catalog #C7905-01R
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA: Used in indirect ELISA or as a detection antibody in a sandwich ELISA with C7905-01Q as a capture antibody. The sandwich ELISA is only suitable for the detection of rat and not mouse C-peptide I or II.
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary AntibodiesI1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo
I1904-65W: IgG, F(abí)2 (HRP) Pab Rb xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B353
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenFragments of rat C-peptide conjugated to a carrier protein.
SpecificityRecognizes an epitope in the N-terminal region of rat C-peptide I and II with almost equal affinity. Species Crossreactivity: mouse. Reacts very weakly with rat proinsulin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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