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You are here:Home » Antibodies » Abs to Hormones, Steroids » Anti -C-Peptide II (Insulin-2, Ins2)

Anti -C-Peptide II (Insulin-2, Ins2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
C-peptide is a 31 amino acid peptide released when proinsulin is split into insulin and C-peptide. In rats, two isoforms of proinsulin exists, the difference is two amino acid residues in the C-peptide, termed C-peptide I and C-peptide II. Proinsulin is split when it is released from the pancreas into the blood in response to a rise in serum glucose levels. C-peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2+ concentration and subsequent stimulation of N+, K+-ATPase and endothelial nitric oxide synthase activities. C-peptide also functions in repair of the muscular layer of arteries.
Catalog #C7905-26
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B357
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenFragments of rat C-peptide conjugated to a carrier protein.
SpecificityRecognizes rat C-peptide II. Species Crossreactivity: mouse. Does not recognize rat C-peptide I and reacts very weakly with mouse C-peptide I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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