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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -CPI-17 (PKC Potentiated Inhibitor Protein-17)

Anti -CPI-17 (PKC Potentiated Inhibitor Protein-17)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17kD protein. Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold. Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity.
Catalog #C7905-50
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.2-1ug/ml. Detects CPI-17 in L6 lysates. L6 lystate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7905-50. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourcePorcine
Concentration~1mg/ml
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 10% glycerol
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to full length porcine CPI-17.
SpecificityRecognizes porcine CPI-17 at ~22kD.
Species CrossreactivityHuman, mouse, rat, bovine and rabbit. Does not crossreact with yeast.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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