| PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17kD protein. Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold. Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity. |
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| Catalog # | C7905-50 |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.2-1ug/ml. Detects CPI-17 in L6 lysates. L6 lystate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7905-50. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Porcine |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, sodium azide, 10% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant protein, full length porcine CPI-17. |
| Specificity | Recognizes porcine CPI-17 at ~22kD. |
| Species Crossreactivity | Human, mouse, rat, bovine, rabbit. Does not crossreact with yeast. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
