Anti -CRACC (SLAMF7, SLAM family member 7, 19A, CSI, CD2-like receptor-activating cytotoxic cells, CD2 subset 1, CD319, CS1, Membrane protein FOAP-12, Novel Ly9, Protein 19A, UNQ576/PRO1138)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E B |
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| CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to | | | the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of | | | CD8 + cells, CD4 + cells and B cells. CRACC may play a role in the activation and effector | | | function of T cells and NK cells. | | | Catalog # | C7906-72B | | Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample | | buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml antibody and chemiluminescent detection substrate. | | ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is approximately 1ng/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 9H101 | | Host | Rat | | Source | Mouse | | Concentration | ~500ug/ml | | Form | Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml. | | | Purity | Purified by Protein G immunoaffinity chromatography. | | Immunogen | Purified, CHO-derived, recombinant mouse CRACC (rmCRACC; aa 22 - 224; Accession# Q8BHK6). | | Specificity | Detects rmCRACC in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross-reactivity with rhCRACC, rmCD229, rmCD84, or rmNTB-A. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -CRACC (SLAMF7, SLAM family member 7, 19A, CSI, CD2-like receptor-activating cytotoxic cells, CD2 subset 1, CD319, CS1, Membrane protein FOAP-12, Novel Ly9, Protein 19A, UNQ576/PRO1138) |
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