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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E IH IC

Cranin is expressed in brain and many other tissues, it binds laminin with high affinity in a calcium dependent manner. It appears to be important in maintaining normal muscle integrity. Loss of cranin from the muscle surface may be one of the primary events leading to muscle injury in congenital muscular dystrophies, it may also play a role in the cognitive deficits often seen in such conditions.
Catalog #	C7906-75
Applications	Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Not recommended for use in Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:1-1:50 detects Cranin on tissue fixed briefly with 0.5% glutaraldehyde, 4% paraformaldehyde, methanol or acetone. Prolonged fixation should be avoided.
	Immunocytochemistry: Neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	2Q36
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Ascites
Immunogen	Synthetic peptide corresponding to aa572-604 of human cranin.
Specificity	Recognizes human Cranin (dystroglycan), extracellular site, near the C-terminus of the alpha-subunit. The expression and localization of cranin varies considerably according to the cells physiologic state and differentiaion state. Recognizes the same peptide region as C7906-76, but a different isotope. Species crossreactivity: rat, mouse, sheep, chicken, Xenopus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.