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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » C-Reactive Protein, Human (CRP, MGC88244, PTX 1, PTX1) BioAssay™ ELISA Kit

C-Reactive Protein, Human (CRP, MGC88244, PTX 1, PTX1) BioAssay™ ELISA Kit

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Specifications

Catalog #C7907-01
PrincipleC-Reactive Protein, Human (CRP) BioAssay™ ELISA Kit is to be used for the in vitro quantitative determination of human C Reactive Protein (CRP) concentrations in serum and plasma. This kit is based on enzyme-linked immunosorbent assay (ELISA) which applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for CRP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. CRP, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound CRP and other components of sample. In order to quantitate the amount of CRP present in the sample, a standardized preparation ofantibody specific for human CRP labeled with horseradish peroxidase (HRP) is added to each well to "sandwich" the CRP immobilized during the second incubation. The wells are thoroughly washed to remove all unbound HRP-labeled antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-labeled antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution. The color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of CRP in the samples, the kit standards (ready-to use) are assayed at the same time as the samples (diluted if necessary with Sample Diluent). This allows the operator to produce a standard curve of Optical Density (O.D.) versus CRP concentration (ug/ml). The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
SensitivityMinimum detectable dose of CRP is 0.03ug/ml
RangeSerum samples: 0.25-1.5ug/ml
Plasma samples: 0.25-1.75ug/ml
Kit ComponentsC7907-01A: CRP Microtiter Plate, 1x96wells
C7907-01B: Pab (HRP), 1x12ml
Anti-human CRP labeled with HRP.
C7907-01C: CRP Standard 2ug/ml, 1x1ml
C7907-01D: CRP Standard 1ug/ml, 1x1ml
C7907-01E: CRP Standard 0.5ug/ml, 1x1ml
C7907-01F: CRP Standard 0.25ug/ml, 1x1ml
C7907-01G: CRP Standard 0.125ug/ml, 1x1ml
C7907-01H: CRP Standard 0.0625ug/ml, 1x1ml
C7907-01J: CRP Standard 0.031ug/ml, 1x1ml
C7907-01K: CRP Standard 0ug/ml, 1x1ml
C7907-01L: Wash Buffer, 20X, 1x60ml
C7907-01M: Substrate A, H2O2 1x10ml
C7907-01N: Substrate B, TMB 1x10ml
C7907-01P: Stop Solution, H2SO4 1x14ml
C7907-01Q: Sample Diluent, 1x30ml
Storage and StabilityStore components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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