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You are here:Home » Molecular Biology » MB-Cardiac Markers » CRP, Western Blot Positive Control (C-Reactive Protein, MGC88244, PTX 1, PTX1)

CRP, Western Blot Positive Control (C-Reactive Protein, MGC88244, PTX 1, PTX1)

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Specifications

Positive Control for C7907-21H.
Catalog #C7907-21N
Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein (CRP or PTX1; mature chain 206aa; chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation; in the case of CRP the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500-molecular weight subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver; those cells produce CRP detectable on lymphocytes.
SourceHuman CRP was purified from human serum/fluids.
ApplicationsSuitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required. Other applications not tested.
Recommended DilutionWestern Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman serum/fluids
Purity~95% (RID, SDS-PAGE, IEP)
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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