Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Antibodies-Enzymes, Creatine Kinase » Anti -CK-BB, NT (CKBB, Creatine Kinase B-type, B-CK, Creatine Kinase B Chain, CKB)

Anti -CK-BB, NT (CKBB, Creatine Kinase B-type, B-CK, Creatine Kinase B Chain, CKB)


  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Human CKB is a protein of 381aa (~45kD), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.
Catalog #C7910-16H
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50 (reduced; size ~43-46kD)
Immunohistochemistry (Frozen): fixed 2hr in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1M PB; stored until use in 1% paraformaldehyde; blocking with 1% normal swine serum
Immunohistochemistry (Paraffin): antigen retrieval: 0.5% pepsin in 0.01N HCl for 15min at 37°C; endogenous peroxidise blocking by using 3% H2O2 in PBS for 30min; blocking with 1% normal swine serum for 30min.
Optimal dilutions to be determined by the researcher.
Positive Control Human Purkinje cells
Negative Control Connective tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No13A41
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa4-20 SNSHNALKLRFPAEDEF from human creatine kinase brain-type, at N-terminal.
SpecificityRecognizes human creatine kinase B-type. Species Crossreactivity: rabbit, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

External Links