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You are here:Home » Antibodies » Abs to Enzymes, Creatine Kinase » Anti -Creatine Kinase, Muscle (CKMM)

Anti -Creatine Kinase, Muscle (CKMM)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Creatine kinase (CK) is a 40kD cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #C7910-28J
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H104
HostMouse
SourceHuman
Concentration~500ug/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human cardiac muscle P06732.
SpecificityRecognizes human CKMM.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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