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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Creatinine BioAssay™ Kit

Creatinine BioAssay™ Kit

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Specifications

Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes. The Creatinine Assay Kit provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (lambda-max = 570nm) and fluorescence (Ex/Em = 538/587nm). Unlike the picric acid assay, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.
Catalog #C7911-50
Kit ComponentsC7911-50A: Creatinine Assay Buffer 1x25ml
C7911-50B: Creatinine Probe 1x200ul in DMSO
*C7911-50C: Creatinase lyophilized 1x1vial
*C7911-50D: Creatininase lyophilized 1x1vial
*C7911-50E: Creatinine Enzyme Mix lyophilized 1x1vial
*C7911-50F: Creatinine (10umol) lyophilized 1x1vial
Storage and StabilityStore all componenets at -20°C. Reconstituted *C7911-50C, *C7911-50D, *C7911-50E, *C7911-50F stable for 2 months.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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