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Anti -CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)
Anti -CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms. | | | Catalog # | C7915-05C | | Applications | Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:500-1:2000. Detects bands of 46 and 43kD corresponding to phosphorylated CREB. | | Immunohistochemistry (FFPE): 20ug/ml | | ELISA: 1:1000-1:6000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.05mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa122-147, QKRREILSRRPpSYRKILNDLSSDAP, from human CREB Phosphorylated at pS133 (KLH). | | Specificity | Recognizes phosphorylated human CREB at pS133. Species Crossreactivity: mouse and rat. Minimal reactivity with non-phosphorylated CREB by Western Blot and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. Assayed against a variety of tisues including fibroblasts and B-cell lysates. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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