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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)

Anti -CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Catalog #C7915-05C
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:2000. Detects bands of 46 and 43kD corresponding to phosphorylated CREB.
Immunohistochemistry (FFPE): 20ug/ml
ELISA: 1:1000-1:6000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa122-147, QKRREILSRRPpSYRKILNDLSSDAP, from human CREB Phosphorylated at pS133 (KLH).
SpecificityRecognizes phosphorylated human CREB at pS133. Species Crossreactivity: mouse and rat. Minimal reactivity with non-phosphorylated CREB by Western Blot and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. Assayed against a variety of tisues including fibroblasts and B-cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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