| The transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including cAMP, membrane depolarization, increased intracellular Ca++, and growth and neurotrophic factors (1–3) (Figure 1). Phosphorylation of CREB at Ser133 regulates the ability of CREB to activate transcription when bound at a CRE. Mutation of Ser133 renders CREB nonresponsive to multiple signaling pathways. A variety of protein kinases have been shown to phosphorylate CREB at Ser133 in vitro, including PKA, PKC, CAM kinase II and IV, and p90rsk. Phosphorylation at Ser133 has also been shown to promote interaction with a CREB binding protein, CBP, required for transcriptional activation by CREB, AP-1, and SRF dependent promoters (3,4). CREB appears to play an important role in learning and memory in both flies and mice. Mice lacking CREB exhibit deficiencies in spatial learning tasks, while flies overexpressing and lacking CREB show enhanced or diminished learning (5,6). The CREB (Ser133) Antibody Kit provides reagents and protocols for the rapid analysis of CREB (Ser133) phosphorylation status. The Kit includes a phospho-antibody that recognizes CREB (Ser133) only when phosphorylated at Ser133, a control CREB antibody (phosphorylation-state independent), protein controls and protein markers for western blots, and the HRP |
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| Catalog # | C7915-14 |
| Applications | Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. |
| Kit Components | CREB (48H2) Rabbit Mab (43kD) 1x100 ul |
| Phospho-CREB (Ser133)Mab Rb (43kD) 1x100 ul |
| CREB Control Cell Extracts 1x40ul |
| IgG (HRP) goat x rabbit, 1x50ul |
| Biotin (HRP) goat x Rabbit 1x100ul |
| 20X LumiGLO® Reagent and 20X Peroxide 1x5ml |
| Biotinylated Protein Ladder 1x100ul |
| Recommended Dilution | Western blotting: 1:1000 |
| Immunoprecipitation: 1:250 |
| Immunocytochemistry: 1:1000 |
| Immunohistochemistry: 1:100 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Purity | Purified by protein A and peptide affinity chromatography. |
| Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
