| CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9). |
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| Catalog # | C7915-25 |
| Applications | Suitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 1:1000 |
| Immunoprecipitation: 1:250 |
| Chromatin Immunoprecipitation: 1:50 |
| Immunohistochemistry (Paraffin): 1:400; Use citrate as an unmasking buffer. |
| Immunohistochemistry (Frozen): 1:400 |
| Immunofluorescence: 1:1600 |
| Flow Cytometry: 1:200 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9B146 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide. |
| Purity | Supernatant |
| Immunogen | GST-CREB full length fusion protein |
| Specificity | Recognizes endogenous levels of total human CREB protein at ~43kD. Species Crossreactivity: mouse, rat, monkey, D. melanogaster. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
