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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -CREB (cAMP Response Element Binding Protein)

Anti -CREB (cAMP Response Element Binding Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC IF
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).
Catalog #C7915-25
ApplicationsSuitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:250
Chromatin Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:400; Use citrate as an unmasking buffer.
Immunohistochemistry (Frozen): 1:400
Immunofluorescence: 1:1600
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9B146
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
PuritySupernatant
ImmunogenGST-CREB full length fusion protein
SpecificityRecognizes endogenous levels of total human CREB protein at ~43kD. Species Crossreactivity: mouse, rat, monkey, D. melanogaster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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