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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH IC IF

Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is a homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.
Catalog #	C7920
Applications	Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:500-1:5000
	Western Blot (Native & recombinant protein): 1:500-1:5000
	Immunohistochemistry: 1:500-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	2Q2151
Host	Mouse
Concentration	Not Determined
Form	Supplied as a liquid. No preservatives added.
Purity	Ascites
Immunogen	Cre recombinase aa77-343 fusion protein.
Specificity	Recognizes both native and recombinant Cre recombinase at ~38kD from all species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.