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You are here:Home » Antibodies » Abs to Enzymes » Anti -Cre Recombinase

Anti -Cre Recombinase

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IH IC IF
Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is a homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.
Catalog #C7920
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsELISA: 1:500-1:5000
Western Blot (Native & recombinant protein): 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q2151
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid. No preservatives added.
PurityAscites
ImmunogenCre recombinase aa77-343 fusion protein (NM_004380).
SpecificityRecognizes both native and recombinant Cre recombinase at ~38kD from all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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