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You are here:Home » Antibodies » Abs to Disease Markers » Anti -CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

Anti -CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
CrkL is a widely-expressed 39kD adaptor protein that belongs to the Crk protein family and is
abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL
initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G,
SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and
the immune response. Furthermore, this adaptor protein plays an essential role in Chronic
Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #C7925-03B
Recommended DilutionWestern Blot:1ug/ml. Lysates from human K562, A431 and Jurkat cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml mouse anti-CrkL
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H105
HostMouse
SourceHuman
Concentration~500ug/ml
FormSupplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
PurityPurified by Protein G immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived full length recombinant human CrkL (rhCrkL; aa 1-303; Accession# P46109).
SpecificityDetects endogenous human, mouse and rat CrkL at 39kD using Western blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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