| CrkL is a widely-expressed 39kD adaptor protein that belongs to the Crk protein family and is |
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| abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL |
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| initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, |
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| SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and |
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| the immune response. Furthermore, this adaptor protein plays an essential role in Chronic |
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| Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase. |
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| Applications: |
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| Suitable for use in Western Blot. Other applications not tested. |
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| Catalog # | C7925-03B |
| Recommended Dilution | Western Blot:1ug/ml. Lysates from human K562, A431 and Jurkat cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml mouse anti-CrkL |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 9H105 |
| Host | Mouse |
| Source | Human |
| Concentration | ~500ug/ml |
| Form | Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml. |
| Purity | Purified by Protein G immunoaffinity chromatography. |
| Immunogen | Purified, E. coli-derived full length recombinant human CrkL (rhCrkL; aa 1-303; Accession# P46109). |
| Specificity | Detects endogenous human, mouse and rat CrkL at 39kD using Western blot. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
