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You are here:Home » Antibodies » Abs to Disease Markers » Anti -CrkL (CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like)

Anti -CrkL (CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian
sarcoma virus CT10 oncogene homolog-like)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #C7925-10A
Alternate Name CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like
Domain Information SH2 domain; SH3 domain; Src homology 2 domains; Src homology 3 domains
Gene Ontology Terms JNK cascade; oncogenesis; cell motility; signal transduction; SH3/SH2 adaptor protein; protein tyrosine kinase; protein phosphorylation; RAS protein signal transduction; intracellular signalling cascade
Application(s)Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 37kD band observed in K562 and Jurkat lysates (calculated MW of 33.8kD according to NP_005198). Recommended concentration: 0.01-0.03ug/ml.
0.01ug/ml staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See C7925-10A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenC-KIFDPQNPDENE, C Terminus
SpecificityRecognizes Human, Mouse, Rat CRKL
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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