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You are here:Home » Antibodies » Abs to Disease Markers » Anti -CrkL, phosphorylated (Tyr207) (Crk-like Protein, CRKL)

Anti -CrkL, phosphorylated (Tyr207) (Crk-like Protein, CRKL)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
CrkL is a 39kD adaptor protein, originally cloned in proximity to the BCR gene on chromosome 22, which has a key regulatory role in hematopoietic cells. CrkL has one SH2 and two SH3 domains, with 60% homology to CrkII. Structurally, the amino-terminal SH3 domain of CrkL has been shown to bind proteins such as C3G, SOS, PI3K, c-Abl or BCR/Abl. The SH2 domain of CrkL can bind to tyrosine phosphorylated proteins such as Cbl, HEF1, CAS or paxillin. CrkL is involved in various signalings initiated by different cytokines and growth factors. The biological outcomes of the Crk-activated signal transduction cascades include the modulation of cell adhesion, cell migration, immune cell responses and so on. CrkL is a prominent substrate of the BCR/Abl oncoprotein in chronic myelogenous leukemia and binds to both BCR/Abl and c-Abl. CrkL is prominently and constitutively tyrosine phosphorylated in CML neutrophils and is not phosphorylated in normal neutrophils. Moreover, stimulation of normal neutrophils with cytokines and agonists does not induce tyrosine phosphorylation of this protein, indicating that it may be a useful target for therapeutic intervention or as a disease marker. Tyrosine 207 in CrkL is the BCR/Abl phosphorylation site.
Catalog #C7925-50C
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:4000
Western Blot: 1:500-1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human CrkL around the phosphorylation site of tyrosine 207 (H-A-YP-A-Q).
SpecificityRecognizes human CrkL when phosphorylated at tyrosine 207. Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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